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Nanobiotechnology is a proficient technology that concers with nano scale materials in several scientific fields such as nanotechnology, chemistry, medicines and biotechnology. A swift and sustainable synthesis of metal oxide nanoparticles by exhausting natural sources is the present-day trend being used instead of lethal biochemical methods. This study prsent the first report for the use of medicinal plant of Conocarpus erectus as an ecofriendly regent in the synthesis of CuO NPs using copper sulphate as a starting material. Conocarpus erectus leaf extract was used as a bioreductor as wll as a capping agent in the manufacturing of MgO NPs. The structure and morphology of prepared MgO NPs were characterized byXRD, UV, FTIR, HRTEM, SEM and EDX analysis. The UV-Vis measurment confimrd the formation of MgO NPs by giving SPR peak at 261 nm. The attendance of various functional groups in Conocarpus erectus leaf extract liable for the development and stabilization of MgO NPs were confirmed by FTIR analysis. XRD measurement confirmed the crystaline f.c.c structure of MgO NPs. HRTEM and SEM studies exhibited the small size, spherical shape and uniformly dispersed magnesium oxide nanoparticles. EDX profile further authorized the elemental composition of the synthesized nanoparticles. The biosynthesized MgO NPs were assessed for the embarrassment of bacteria. Furthermore, plant capped MgO NPs have high inhibition efficiency against S. aureus and E. coli with inhibition zones of 17 mm and 16 mm respectively.